71 research outputs found

    An improved stability criterion for discrete-time time-delayed Lur’e systemwith sector-bounded nonlinearities

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    The absolute stability problem of discrete-time time-delayed Lur\u27e systems with sector bounded nonlinearities is investigated in this paper. Firstly, a modified Lyapunov-Krasovskii functional (LKF) is designed with augmenting additional double summation terms, which complements more coupling information between the delay intervals and other system state variables than some previous LKFs. Secondly, some improved delay-dependent absolute stability criteria based on linear matrix inequality form (LMI) are proposed via the modified LKF and the relaxed free-matrix-based summation inequality technique application. The stability criteria are less conservative than some results previously proposed. The reduction of the conservatism mainly relies on the full use of the relaxed summation inequality technique based on the modified LKF. Finally, two common numerical examples are presented to show the effectiveness of the proposed approach

    Chlorimuronethyl Resistance Selectable Marker Unsuited for the transformation of rice blast fungus (Magnaporthe grisea)

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    Abstract: Chlorimuronethyl resistance gene is increasingly used as a selectable marker for transformation, especially fungal transformation. Magnaporthe grisea is an important model organism for investigating fungal pathogenicity, and Agrobacterium tumefaciens-mediated transformation (ATMT) is used for functional mutagenesis of the fungus. However, our results showed that rice blast strains collected from infectious rice fields have highly conserved resistance to chlorimuronethyl, even comparable to transformants which carrying chlorimuronethyl resistance genes as selectable marker in laboratory conditions. PCR results showed that all tested field strains presented the amplified products of the same size as the selectable marker amplified from plasmid carrying chlorimuronethyl gene. Sequence analysis of PCR products amplified from field strains confirmed that field strains harbored the highly identity homolog of chlorimuronethyl resistance gene. Blast search in GenBank suggested that the fragment is presenting in reference genome sequence of 70-15, but it is not a wide-spread gene in other organisms, excepted for Herpetosiphon aurantiacus. Although the origin and reason of the conserved chlorimuronethyl resistance gene in field isolates of blast fungus is unclear, the ecological function of the gene is noteworthy

    Crop Diversity for Yield Increase

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    Traditional farming practices suggest that cultivation of a mixture of crop species in the same field through temporal and spatial management may be advantageous in boosting yields and preventing disease, but evidence from large-scale field testing is limited. Increasing crop diversity through intercropping addresses the problem of increasing land utilization and crop productivity. In collaboration with farmers and extension personnel, we tested intercropping of tobacco, maize, sugarcane, potato, wheat and broad bean – either by relay cropping or by mixing crop species based on differences in their heights, and practiced these patterns on 15,302 hectares in ten counties in Yunnan Province, China. The results of observation plots within these areas showed that some combinations increased crop yields for the same season between 33.2 and 84.7% and reached a land equivalent ratio (LER) of between 1.31 and 1.84. This approach can be easily applied in developing countries, which is crucial in face of dwindling arable land and increasing food demand

    Parameter Optimization of Support Vector Machine to Improve the Predictive Performance for Determination of Aflatoxin B<sub>1</sub> in Peanuts by Olfactory Visualization Technique

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    This study proposes a novel method for detection of aflatoxin B1 (AFB1) in peanuts using olfactory visualization technique. First, 12 kinds of chemical dyes were selected to prepare a colorimetric sensor to assemble olfactory visualization system, which was used to collect the odor characteristic information of peanut samples. Then, genetic algorithm (GA) with back propagation neural network (BPNN) as the regressor was used to optimize the color component of the preprocessed sensor feature image. Support vector regression (SVR) quantitative analysis model was constructed by using the optimized combination of characteristic color components to achieve determination of the AFB1 in peanuts. In this process, the optimization performance of grid search (GS) algorithm and sparrow search algorithm (SSA) on SVR parameter was compared. Compared with GS-SVR model, the model performance of SSA-SVR was better. The results showed that the SSA-SVR model with the combination of seven characteristic color components obtained the best prediction effect. Its correlation coefficients of prediction (RP) reached 0.91. The root mean square error of prediction (RMSEP) was 5.7 μg·kg−1, and ratio performance deviation (RPD) value was 2.4. The results indicate that it is reliable to use the colorimetric sensor array with strong specificity for the determination of the AFB1 in peanuts. In addition, it is necessary to properly optimize the parameters of the prediction model, which can obviously improve the generalization performance of the multivariable model

    High Precisive Prediction of Aflatoxin B1 in Pressing Peanut Oil Using Raman Spectra Combined with Multivariate Data Analysis

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    This study proposes a label-free rapid detection method for aflatoxin B1 (AFB1) in pressing peanut oil based on Raman spectroscopy technology combined with appropriate chemometric methods. A DXR laser Raman spectrometer was used to acquire the Raman spectra of the pressed peanut oil samples, and the obtained spectra were preprocessed by wavelet transform (WT) combined with adaptive iteratively reweighted penalized least squares (airPLS). The competitive adaptive reweighted sampling (CARS) method was used to optimize the characteristic bands of the Raman spectra pretreated by the WT + airPLS, and a partial least squares (PLS) detection model for the AFB1 content was established based on the features optimized. The results obtained showed that the root mean square error of prediction (RMSEP) and determination coefficient of prediction (RP2) of the optimal CARS-PLS model in the prediction set were 22.6 &micro;g/kg and 0.99, respectively. The results demonstrate that the Raman spectroscopy combined with appropriate chemometrics can be used to quickly detect the safety of edible oil with high precision. The overall results can provide a technical basis and method reference for the design and development of the portable Raman spectroscopy system for the quality and safety detection of edible oil storage, and also provide a green tool for fast on-site analysis for regulatory authorities of edible oil and production enterprises of edible oil
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